Affiliation:
1. Computation and Simulation Unit (Analytical and Environmental Science Division and Centralized Instrument Facility) CSIR–Central Salt and Marine Chemicals Research Institute Bhavnagar Gujarat India
2. Academy of Scientific and Innovative Research Ghaziabad India
Abstract
AbstractDuring the last decade, riboswitches emerged as new small‐molecule sensing RNA in bacteria. Thiamine pyrophosphate (TPP) riboswitch is widely distributed and occurs in plants, bacteria, fungi, and archaea. Extensive biochemical, structural, and genetic studies have been carried out to elucidate the recognition mechanism of TPP riboswitches. However, a comprehensive report summarizing all information on recognition principles and newly designed ligands for TPP riboswitch is scarce in the literature. This review gives a comprehensive understanding of the TPP riboswitch's structure, mechanism, and methods applied to design ligands for the TPP riboswitch. The ligand‐bound TPP riboswitch was studied with various experimental and theoretical techniques to elucidate the conformational dynamics. The mutation studies shed light on the significance of pyrimidine sensing helix for the binding of ligands. Further, the structure–activity relationship study and fragment‐based approach lead to the development of ligands with Kd values at the sub‐micromolar level. However, there is a need to design more potent inhibitors for TPP riboswitch for therapeutic applications. The recent advancements in ligand design highlight the TPP riboswitch as a promising target for developing new antibiotics.This article is categorized under:
Regulatory RNAs/RNAi/Riboswitches > Riboswitches
Regulatory RNAs/RNAi/Riboswitches > Regulatory RNAs
Subject
Molecular Biology,Biochemistry
Cited by
2 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献