International Society for Extracellular Vesicles workshop. QuantitatEVs: Multiscale analyses, from bulk to single extracellular vesicle

Author:

Basso Manuela1ORCID,Gori Alessandro2,Nardella Caterina1,Palviainen Mari3ORCID,Holcar Marija4,Sotiropoulos Ioannis5,Bobis‐Wozowicz Sylwia6ORCID,D'Agostino Vito G.1ORCID,Casarotto Elena7,Ciani Yari1,Suetsugu Shiro8,Gualerzi Alice9ORCID,Martin‐Jaular Lorena10ORCID,Boselli Daniela11,Kashkanova Anna12,Parisse Pietro13,Lippens Lien1415,Pagliuca Martina1617,Blessing Martin12,Frigerio Roberto2,Fourniols Thibaut18,Meliciano Ana19,Fietta Anna2021,Fioretti Paolo Vincenzo1,Soroczyńska Karolina22,Picciolini Silvia9,Salviano‐Silva Amanda23,Bergese Paolo2425,Zocco Davide26,Chiari Marcella2,Jenster Guido27,Waldron Levi28,Milosavljevic Aleksandar29,Nolan John30,Monopoli Marco P.31,Witwer Kenneth W.32ORCID,Bussolati Benedetta33ORCID,Di Vizio Dolores34,Falcon Perez Juan353637ORCID,Lenassi Metka4ORCID,Cretich Marina2ORCID,Demichelis Francesca1ORCID

Affiliation:

1. Department of Cellular, Computational, and Integrative Biology (CIBIO) University of Trento Trento Italy

2. National Research Council of Italy Istituto di Scienze e Tecnologie Chimiche (SCITEC‐CNR) Milan Italy

3. EV group, Molecular and Integrative Biosciences Research Program, Faculty of Biological and Environmental Sciences University of Helsinki Helsinki Finland

4. Institute of Biochemistry and Molecular Genetics, Faculty of Medicine University of Ljubljana Ljubljana Slovenia

5. Institute of Biosciences & Applications National Center for Scientific Research (NCSR) Demokritos Paraskevi Greece

6. Faculty of Biochemistry, Biophysics and Biotechnology, Department of Cell Biology Jagiellonian University Krakow Poland

7. Dipartimento di Scienze Farmacologiche e Biomolecolari “Rodolfo Paoletti” (DiSFeB), Dipartimento di Eccellenza Università degli Studi di Milano Milan Italy

8. Division of Biological Science Graduate School of Science and Technology, Nara Institute of Science and Technology Ikoma Japan

9. IRCCS Fondazione Don Carlo Gnocchi Milan Italy

10. Institut Curie PSL University, Inserm U932, Immunity and Cancer Paris France

11. FRACTAL (Flow Cytometry Resource, Advanced Cytometry Technical Applications Laboratory) San Raffaele Scientific Institute Milan Italy

12. Max Planck Institute for the Science of Light Erlangen Germany

13. National Research Council of Italy, Istituto Officina dei Materiali (IOM‐CNR) Trieste Italy

14. Department of Human Structure and Repair, Laboratory of Experimental Cancer Research Ghent University Ghent Belgium

15. Cancer Research Institute Ghent Ghent Belgium

16. Molecular Predictors and New Targets in Oncology Gustave Roussy Villejuif France

17. Clinical and Translational Oncology Scuola Superiore Meridionale Naples Italy

18. EverZom Paris France

19. iBET‐Instituto de Biologia Experimental e Tecnológica Oeiras Portugal

20. Department of Biomedical Sciences (DSB) University of Padua Padua Italy

21. Fondazione Istituto di Ricerca Pediatrica Città della Speranza (IRP) Padua Italy

22. Medical University of Warsaw Warszawa Poland

23. Department of Neurosurgery University Medical Center Hamburg‐Eppendorf Hamburg Germany

24. Department of Molecular and Translational Medicine Università degli Studi di Brescia Brescia Italy

25. IRIB ‐ Institute for Research and Biomedical Innovation of CNR Palermo Italy

26. Lonza Siena Siena Italy

27. Department of Urology, Erasmus MC Cancer Institute Erasmus University Medical Center Rotterdam The Netherlands

28. Graduate School of Public Health and Health Policy City University of New York New York New York USA

29. Department of Molecular and Human Genetics, Dan L Duncan Comprehensive Cancer Center, and Program in Quantitative and Computational Biosciences Baylor College of Medicine Houston Texas USA

30. Scintillon Institute San Diego California USA

31. Department of Chemistry RCSI Dublin Ireland

32. Department of Molecular and Comparative Pathobiology Johns Hopkins University School of Medicine Baltimore Maryland USA

33. Department of Molecular Biotechnology and Health Sciences University of Turin Turin Italy

34. Department of Surgery, Division of Cancer Biology and Therapeutics Cedars‐Sinai Medical Center Los Angeles California USA

35. Center for Cooperative Research in Biosciences (CIC bioGUNE) Basque Research and Technology Alliance (BRTA), Exosomes Laboratory Derio Spain

36. Centro de Investigación Biomédica en Red de enfermedades hepáticas y digestivas (CIBERehd) Madrid Spain

37. IKERBASQUE, Basque Foundation for Science Bilbao Spain

Abstract

AbstractThe “QuantitatEVs: multiscale analyses, from bulk to single vesicle” workshop aimed to discuss quantitative strategies and harmonized wet and computational approaches toward the comprehensive analysis of extracellular vesicles (EVs) from bulk to single vesicle analyses with a special focus on emerging technologies. The workshop covered the key issues in the quantitative analysis of different EV‐associated molecular components and EV biophysical features, which are considered the core of EV‐associated biomarker discovery and validation for their clinical translation. The in‐person‐only workshop was held in Trento, Italy, from January 31st to February 2nd, 2023, and continued in Milan on February 3rd with “Next Generation EVs,” a satellite event dedicated to early career researchers (ECR). This report summarizes the main topics and outcomes of the workshop.

Funder

Cancer Research UK

Publisher

Wiley

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