Unveiling thrips morphology: A comparative analysis of microscopy‐based ultrastructural, morphological, and molecular characterization of Thrips tabaci and Thrips parvispinus in onion

Author:

Pandi Arunkumar1ORCID,Malaichamy Kannan1ORCID,John Samuel Kennedy1,Selvaraj Dineshkumar1,Kasivelu Govindaraju2ORCID,Kolanthasamy Elango3ORCID

Affiliation:

1. Department of Agricultural Entomology Tamil Nadu Agricultural University Coimbatore India

2. Centre for Ocean Research Sathyabama Institute of Science and Technology Chennai India

3. Kumaraguru Institute of Agriculture Erode India

Abstract

AbstractThe present study unveils the intricate details on the morphology of thrips through optical, field emission scanning electron microscopy (FE‐SEM) and mitochondrial cytochrome oxidase I gene‐based molecular identification tools. The variation in the morphological characters namely, antennae (seven‐segmented with forked sensorium on third, fourth segments), ctenidia (paired ctenidia were present in 5th–8th abdominal segments laterally), pronotum (two pairs of posteroangular setae) were observed in both Thrips tabaci and Thrips parvispinus, respectively. Similarly, ocelli color (brown and red colored), ocellar setae (two and three pairs of ocellar setae on the head of T. tabaci and T. parvispinus, respectively. Irregular reticulate striations on metascutum and medial striations are present in the metanotum of T. parvispinus; forewings with 6 distal setae in the first vein and 15 distal setae in the second vein in T. tabaci and forewings of T. parvispinus with complete rows of setae in the first and second vein in T. parvispinus; abdomen with median dorsal setae present in the tergite of T. tabaci and presence of 6–12 discal setae in sternites III–VI segments, absence of discal setae on sternites II and VII in T. parvispinus were observed, respectively. Further, FE‐SEM studies revealed that similar type of sensilla namely, sensilla basiconica (SBI, SBII, SBIII), sensilla chaetica (SChI, SChII), sensilla trichodea (ST), sensilla campaniformia (SCa), and sensilla cavity (SCav) were recorded in both the species and variations were observed in length of above sensilla of T. tabaci and T. parvispinus. Additionally, Bohm bristles (Bb) and microtrichia (Mt) on the antennal surface contributed to a comprehensive understanding of their ultrastructural features. The molecular characterization revealed a single ~450 bp nucleotide fragment with over 98% similarity for the confirmation of T. tabaci and T. parvispinus in concurrence with NCBI data.Research Highlights Microscopy‐based morphological and ultrastructural characterization of Thrips tabaci Lindeman and Thrips parvispinus Karny.

Publisher

Wiley

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3