Affiliation:
1. College of Chinese Medicinal Materials Jilin Agricultural University Changchun China
2. Jilin Provincial Engineering Research Center for Efficient Breeding and Product Development of Sika Deer Jilin Agricultural University Changchun China
3. Key Laboratory of Animal Production and Product Quality and Safety, Ministry of Education Jilin Agricultural University Changchun China
Abstract
AbstractThe role of polysaccharide components in the immune system, especially immunomodulatory effects, has received increasing attention. In this context, in this study, network pharmacology was adopted to explore the hypothesis of a multitarget mechanism for immune modulation by Chrysalis polysaccharides. A total of 174 common targets were screened by network pharmacology, with the main ones being TNF, MAPK3, CASP3, VEGFA, and STAT3, mostly enriched in the Toll pathway. The molecular docking results showed that the polysaccharide fraction of Chrysalis binds well to TNF proteins. Besides, in vitro cellular assays were performed to verify the ability of Chrysalis polysaccharides to regulate macrophage polarization and to screen for macrophage surface receptors. Furthermore, in vivo experiments were conducted to prove the activation of TLR4 and TNF‐α protein expression in mice by Chrysalis polysaccharide.
Subject
Health, Toxicology and Mutagenesis,Toxicology,Molecular Biology,Molecular Medicine,Biochemistry,General Medicine