Prevalence and antimicrobial resistance of Campylobacter species in shelter‐housed healthy and diarrheic cats and dogs in Turkey

Author:

Yildiz Mustafa1,Sahin Orhan2,Adiguzel Mehmet Cemal13ORCID

Affiliation:

1. Faculty of Veterinary Medicine Department of Microbiology Ataturk University Erzurum Turkey

2. Department of Veterinary Diagnostic and Production Animal Medicine College of Veterinary Medicine Iowa State University Ames Iowa USA

3. Veterinary Vaccine and Biological Product Development Application and Research Center Ataturk University Erzurum Turkey

Abstract

AbstractBackgroundCampylobacter spp. are among the leading foodborne bacterial pathogens. Pet animals may be an important reservoir for human infection.ObjectivesTo determine the prevalence and antimicrobial resistance profiles and mechanisms of Campylobacter isolates recovered from shelter‐housed healthy and diarrheic cats and dogs in Erzurum province in Turkey.MethodsA total of 250 rectal swabs (from 124 cats and 126 dogs) collected between 2020 and 2021 were included in this study. The samples were cultured using a Campylobacter‐selective agar medium. A single suspect colony from each plate was purified and species identification was performed by PCR. Minimum inhibitory concentration (MIC) values were determined against eight antibiotics. Specific antimicrobial resistance genes (tetO and aphA‐3) and mutations (in gyrA) were screened by PCR and/or sequencing.ResultsA total of 26 (10.4%) isolates (25 Campylobacter jejuni and 1 Campylobacter coli) were obtained from the dogs; no Campylobacter was isolated from the cats. Of the C. jejuni isolates, 19.2% were resistant to nalidixic acid, 7.7% to ciprofloxacin and 3.8% to tetracycline and gentamicin per the CLSI interpretative criteria. The C. coli isolate was susceptible to all of the tested antibiotics. Thr‐86‐Ile mutation was the most common change detected in the gyrA gene in the quinolone‐resistant isolates.ConclusionWhile geographic and population differences exist, Campylobacter carriage and associated antibiotic resistance in dogs is common, emphasising the need for continuous surveillance in this species, particularly given its zoonotic potential.

Funder

Atatürk Üniversitesi

Publisher

Wiley

Subject

General Veterinary

Reference52 articles.

1. Recovery of thermophilic Campylobacter spp. in healthy and diarrhoeic pets by three culture methods and identification of the isolates by multiplex polymerase chain reaction (mPCR);Abay S.;Kafkas Universitesi Veteriner Fakultesi Dergisi,2014

2. Genetic diversity and antibiotic resistance profiles of Campylobacter jejuni isolates from poultry and humans in Turkey

3. Tetracycline resistance genes in Campylobacter jejuni and C. coli isolated from poultry carcasses;Abdi‐Hachesoo B.;Jundishapur Journal of Microbiology,2014

4. Campylobacteriosis in dogs and cats: a review

5. Prevalence of thermophilic Campylobacter species in household cats and dogs in Ireland

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