Affiliation:
1. Center for Reproductive Medicine and Endoscopy Sugiyama Clinic Marunouchi Tokyo Japan
2. Centre for Reproductive Medicine and Implantation Research Sugiyama Clinic Shinjuku Tokyo Japan
3. Department of Obstetrics and Gynaecology Juntendo University Faculty of Medicine Tokyo Japan
Abstract
AbstractPurposeTo clarify the mechanisms of intrauterine platelet‐rich plasma (PRP) infusion that support embryo implantation in in vitro fertilization treatment.MethodsBlood and endometrial samples were collected from four infertile women. Human endometrial stromal cells (HESCs) were cultured and passaged equally into four cell culture dishes in each patient. Two were treated with PRP twice, and the other two were treated with vehicle. Subsequently, two cultures with and without PRP were decidualized with 8‐bromoadenosine 3′,5′‐cyclic AMP and progesterone for 5 days.ResultsThe gene expression in undifferentiated or decidualized HESCs with and without PRP was compared. In the microarray analysis, 381 and 63 differentially expressed genes were detected in undifferentiated and decidualized HESCs, respectively. In the undifferentiated HESCs, PRP was found to promote the gene expression associated with cell growth, tissue regeneration, proinflammatory response, and antibiotic effects. In decidualized HESCs, PRP was found to attenuate the gene expression involved in cell proliferation and inflammation by inhibiting the expression of phosphoinositide 3‐kinase signaling.ConclusionsPlatelet‐rich plasma regulates the reprogramming of cell proliferation and inflammation depending on menstrual cycle phases in an appropriate manner, suggesting that PRP has the potential to increase endometrial thickness in the proliferative phase and improve immune tolerance in the secretory phase.
Subject
Cell Biology,Reproductive Medicine
Cited by
3 articles.
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