Identification of key residues modulating the stereoselectivity of nitrile hydratase toward rac-mandelonitrile by semi-rational engineering

Author:

Cheng Zhongyi1,Peplowski Lukasz2,Cui Wenjing1,Xia Yuanyuan1,Liu Zhongmei1,Zhang Jialei1,Kobayashi Michihiko3,Zhou Zhemin1ORCID

Affiliation:

1. Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology; Jiangnan University; Wuxi China

2. Faculty of Physics; Institute of Physics; Astronomy and Informatics; Nicolaus. Copernicus University; Grudziadzka 5 Torun Poland

3. Institute of Applied Biochemistry and the Graduate School of Life and Environmental Sciences; The University of Tsukuba; Tsukuba Ibaraki Japan

Publisher

Wiley

Subject

Applied Microbiology and Biotechnology,Bioengineering,Biotechnology

Reference53 articles.

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2. Synthetic applications of epoxide hydrolases;Archelas;Current Opinion in Chemical Biology,2001

3. The SWISS-MODEL workspace: A web-based environment for protein structure homology modelling;Arnold;Bioinformatics,2006

4. The nitrile-degrading enzymes: Current status and future prospects;Banerjee;Applied Microbiology and Biotechnology,2016

5. Enantioselective hydrolysis of racemic 2-phenylpropionitrile and other (R,S)-2-arylpropionitriles by a new bacterial isolate, Agrobacterium tumefaciens strain d3;Bauer;Applied Microbiology and Biotechnology,1994

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