Identification and Quantification of Monosaccharides from Fungal Cell Walls and Exopolysaccharides by Gas Chromatography Coupled to Mass Spectrometry-Reference-Cited by-同舟云学术

Identification and Quantification of Monosaccharides from Fungal Cell Walls and Exopolysaccharides by Gas Chromatography Coupled to Mass Spectrometry

Published:2023-08 Issue:8 Volume:3 Page:
ISSN:2691-1299
Container-title:Current Protocols
language:en
Short-container-title:Current Protocols

Author:

Millette Pierre‐Guy¹²³⁴,Chabot Josée²³,Sheppard Donald C.¹²³⁵,Le Mauff François²³⁵

Affiliation:

1. Department of Microbiology and Immunology McGill University Montreal Canada

2. Infectious Diseases and Immunity in Global Health Program Research Institute of the McGill University Health Center Montreal Canada

3. McGill Interdisciplinary Initiative in Infection and Immunity Montreal Canada

4. Current affiliation: Faculté de Médecine Université de Montréal Montreal Canada

5. Glyco‐NET Integrated Services Microbial Glycomic Node Montreal Canada

Abstract

AbstractThe fungal cell wall and secreted exopolysaccharides play an important role in the interactions between fungi and their environment. Despite their central role in fungal biology, ecology, and host‐pathogen interactions, the composition of these polymers and their synthetic pathways are not well understood. The protocols presented in this article describe an approach to isolate fungal cell wall polysaccharides and to identify and quantify the monosaccharide composition of these polymers by gas chromatography‐mass spectrometry (GC‐MS). © 2023 The Authors. Current Protocols published by Wiley Periodicals LLC.Basic Protocol: O‐methyl trimethylsilyl monosaccharide derivatives composition analysis by GC‐MSSupport Protocol: Fungal cell wall extraction

Publisher

Wiley

Subject

Medical Laboratory Technology,Health Informatics,General Pharmacology, Toxicology and Pharmaceutics,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Neuroscience

Link

https://currentprotocols.onlinelibrary.wiley.com/doi/pdf/10.1002/cpz1.853

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2. The Fungal Cell Wall

3. Understanding Aspergillus fumigatus galactosaminogalactan biosynthesis: A few questions remain