Affiliation:
1. Shenyang Pharmaceutical University 103 Wenhua Road, Shenyang District Shenyang Liaoning 110016 China
2. School of Pharmacy Shenyang Pharmaceutical University 103 Wenhua Road Shenyang 110016 China
Abstract
ScopeAlzheimer's disease (AD) has been a challenge and hotspot in the field of neuroscience research due to the high morbidity. As we all know, walnut kernel (WK) ingestion has been linked to benefits to brain health and has the function of improving memory. This study follows the AD model induced by scopolamine to reveal the active fractions and substances of walnut in the treatment of AD.Methods and resultsThe histopathological analysis and brain tissue biochemistry assay are revealed the active fractions of WK, and this result determines that walnut kernel organic acids have significant therapeutic effect on AD. The strategy of studying ingredients pointed at lesions is integrated to ascertain the selected brain‐targeted effective substances of WK for blood–brain barrier by ultra‐performance liquid chromatography‐quadrupole/electrostatic field orbitrap mass spectrometry, and a total of eight organic acids are figured out definite absorptivity in rat brains. Finally, the binding interaction between the effective substances and target proteins is analyzed by molecular docking, and the main function related active markers are ascertained as glansreginin A, glansreginic acid, ellagic acid, and ellagic acid 4‐O‐xyloside.ConclusionsThe comprehensive process is helpful to the clinical application of WK as a promising cholinesterase inhibitors for nutritional intervention.
Funder
National Natural Science Foundation of China
Subject
Food Science,Biotechnology
Cited by
3 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献