Affiliation:
1. College of Food Science and Nutritional Engineering China Agricultural University Beijing 100193 China
2. Department of Nutrition and Health China Agricultural University Beijing 100193 China
Abstract
ScopeNorisoboldine (NOR) is a major isoquinoline alkaloid component in the traditional Chinese herbal plant Lindera aggregata (Sims) Kosterm, with previously reported anti‐osteoclast differentiation and antiarthritis properties. However, the roles of NOR on osteoblasts, bone marrow mesenchymal stem cells (BMSCs), and osteoporosis in vivo have never been well established.Methods and resultsThis study investigates the ability of NOR to improve bone formation in vitro and in vivo. Osteoblasts and BMSCs are used to study the effect of NOR on osteogenic and adipogenic differentiation. It finds that NOR promotes osteogenic differentiation of osteoblasts and BMSCs, while inhibiting adipogenic differentiation of BMSCs by reducing the relative expression of peroxisome proliferator‐activated receptor γ (Ppar‐γ) and adiponectin, C1Q and collagen domain containing (Adipoq). Mechanistic studies show that NOR increases osteoblast differentiation through the mechanistic target of rapamycin kinase (mTOR)/ribosomal protein S6 kinase; polypeptide 1 (S6K1) pathway, and treatment with an mTOR inhibitor rapamycin blocked the NOR‐induced increase in mineral accumulation. Finally, the study evaluates the therapeutic potential of NOR in a mouse model of ovariectomy (OVX)‐induced bone loss. NOR prevents bone loss in both trabecular and cortical bone by increasing osteoblast number and phospho‐S6K1 (p‐S6K1) expression in osteoblasts.ConclusionNOR effects in enhancing osteoblast‐induced bone formation via S6K1 pathway, suggesting the potential of NOR in osteoporosis treatment by increasing bone formation.
Funder
Beijing Nova Program
National Natural Science Foundation of China
Chinese Universities Scientific Fund
Cited by
1 articles.
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