Potential Function of 3,5‐Dihydroxy‐4‐Methoxybenzyl Alcohol from Pacific Oyster (Crassostrea gigas) in Brain of Old Mice

Author:

Chen MinYu1,Liu Min1,Chen JingHong1,Liu Xinwei1,Tang LiWei1,Wang Chao2,Yu Ziniu3,Zhang Yang3,Tian Jing1ORCID

Affiliation:

1. Shenzhen Key Laboratory of Marine Biotechnology and Ecology College of Life, Sciences and Oceanography Shenzhen University Shenzhen Guangdong 518055 China

2. Chemical Analysis & Physical Testing Institute Shenzhen Center for Disease Control and Prevention Shenzhen Guangdong 518055 China

3. Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture, Key Laboratory of Tropical Marine Bio‐resources and Ecology and Guangdong Provincial Key Laboratory of Applied Marine Biology South China Sea Institute of Oceanology Chinese Academy of Sciences Guangzhou 510301 China

Abstract

Scope3,5‐Dihydroxy‐4‐methoxybenzyl alcohol (DHMBA) is found in oyster extracts in recent years and is reported to have antioxidant activity. Although it has been reported to be protective in various models of oxidative stress, the therapeutic effect of DHMBA on neurological damage caused by aging remains to be demonstrated.Methods and ResultsThe present study investigates the potential functions of DHMBA in brain of old C57BL/6J mice and aging cell model. Administration of DHMBA improves working memory, reduces anxiety behavior, decreases the expression levels of cell cycle proteins, cycin‐dependent kinase inhibitor 1(P21) and peptidase inhibitor 16(P16)  and inhibits neuronal loss in old mice. The data obtained from the aging cell model are consistent with those from the old mice. The interaction between DHMBA and Kelch‐like ECH‐associated protein 1 (Keap1) is predicted by molecular docking assay, and then it is verified by co‐immunopricipitation (CoIP) that factor red lineage 2‐related factor 2 (Nrf2)‐Keap1 protein‐protein interaction is inhibited by DHMBA. Protein levels of Nrf2 and its target genes, such as glutathione peroxidase 4(GPX4) and heme oxygenase 1 (HO‐1), are detected in old mice and aging cell model.ConclusionThis study provides new evidence that explores the antioxidant mechanism of DHMBA and implies a potential role of DHMBA on antiaging in brain.

Funder

Shenzhen Fundamental Research Program

National Natural Science Foundation of China

Publisher

Wiley

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