Micronutrients in High‐Fat Diet Modify Insulin Resistance and Its Regulatory Genes in Adult Male Mice

Author:

Yang Zeyu1,Kubant Ruslan1,Cho Clara E.2ORCID,Kranenburg Eva1,Beaudry Jacqueline1,Bottiglieri Teodoro3,Anderson G. Harvey14ORCID

Affiliation:

1. Department of Nutritional Sciences Faculty of Medicine University of Toronto Toronto Ontario Canada

2. Department of Human Health and Nutritional Sciences College of Biological Science University of Guelph Guelph Ontario Canada

3. Institute of Metabolic Disease Baylor Scott & White Health Austin TX USA

4. Department of Physiology Faculty of Medicine University of Toronto Toronto Ontario Canada

Abstract

ScopeObesity and insulin resistance (IR) are associated with epigenetic changes of gene expression. However, the relationship between micronutrients, epigenetic regulation of gene expression, and IR during development of diet‐induced obesity has yet to be defined. Our objective is to describe the effect of micronutrient addition to diets on IR and its related genes during obesity development.Methods and resultsMale C57BL/6J mice are fed a high‐fat (HFD) or low‐fat (LFD) diets with or without a multi‐vitamin mineral mix (MVM) addition containing vitamins A, B1, B6, B12, and Zn, and Se for 9 weeks. Compared to LFD mice, HFD mice have higher body weight, IR, fasting glucose, insulin, C‐peptide, leptin, and hepatic triglyceride concentrations, and dysregulated gene expression in liver, muscle, pancreas, and fat tissues (p < 0.05). The addition of MVM reduces these HFD‐induced effects. HFD downregulates 27 genes associated with insulin regulation and adipose tissue function across all tissues by an average of 47% and upregulates five genes by 230% (p < 0.001). Adding MVM downregulates five genes and upregulates one in HFD‐fed mice. Both HFD and MVM alter one‐carbon metabolites.ConclusionAddition of micronutrients to the HFD decreases IR and modifies associated gene expression in obese and lean mice.

Funder

Canadian Institutes of Health Research

Natural Sciences and Engineering Research Council of Canada

Publisher

Wiley

Subject

Food Science,Biotechnology

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