Affiliation:
1. School of Agriculture and Biology Shanghai Jiao Tong University Dongchuan 800 Shanghai 200240 China
2. State Key Laboratory of Dairy Biotechnology, Dairy Research Institute, Bright Dairy and Food Co., Ltd, Shanghai, China Jiangchang West Road 1518 Shanghai 200436 China
Abstract
ScopeConsumption of inulin could affect the intestinal microbiota composition. Hereby, it is aimed to investigate the intestinal microbial community restoration process when the inulin supplementation is terminated (i.e., the secondary effect).Methods and resultsThe current study investigates the response and restoration of intestinal microbiota to/after high (Inulin‐H) and low (Inulin‐L) dosage of inulin supplementation or sequential antibiotics and inulin (Anti‐Inulin‐L) supplementation, based on analysis of 16S rRNA gene sequences in C57BL/6 mice. The number of significantly changed genera in response to inulin is highest in Anti‐Inulin‐L (n = 66) group, followed by Inulin‐H (n = 51) and Inulin‐L (n = 38) group. After inulin supplementation stops, microbiota of all studied groups tend to recover to their original states, with highest percentage of inulin‐responding microbes stay significantly different at Anti‐Inulin‐L (93.94%) group, followed by Inulin‐H (74.51%) and Inulin‐L (44.12%) groups. Of note, the relative abundance of some non‐inulin‐responding taxa significantly increases during restoration.ConclusionSequential antibiotics and inulin supplementation induce greatest changes in the intestinal microbial composition, followed by high and low dosage of inulin. Additionally, the changes induce by supplemented inulin in the intestinal microbial community, provide a chance for some microbes to outcompete the other microbes during the spontaneous restoration.