Affiliation:
1. College of Animal Science and Technology Northwest A&F University Yangling 712100 P. R. China
2. College of Animal Science and Technology Shanxi Agricultural University Taigu 030031 P. R. China
3. School of Life Science and Engineering Southwest University of Science and Technology Mianyang 621000 P. R. China
4. College of Pharmacy Shenzhen Technology University Shenzhen 518118 P. R. China
Abstract
ScopeIsorhamnetin is a natural flavonoid with various pharmacological activities, which can be widely and continuously ingested by humans and animals through their daily diet. The aim of this study is to explore the benefits and molecular mechanisms of isorhamnetin on oocyte maturation.Methods and resultsOocytes are incubated with isorhamnetin (5, 10, 20, and 30 µM) for 44 h. Isorhamnetin (10 µM) increases the polar body extrusion rate of oocytes. Furthermore, isorhamnetin alleviates oxidative stress by inhibiting reactive oxygen species levels and stimulating SOD2 protein expression. The changes in intracellular mitochondrial autophagy and apoptosis‐related proteins (Bcl‐2, Bax/Bcl‐2, and C‐Casp3) indicate that isorhamnetin inhibits oocyte apoptosis. Isorhamnetin inhibits endoplasmic reticulum stress by reducing the protein expression of CHOP and GRP78 and improving the normal distribution rate of endoplasmic reticulum. Mechanistic studies show that isorhamnetin activates the PI3K/Akt signaling pathway.ConclusionIsorhamnetin promotes oocyte maturation by inhibiting oxidative stress, mitochondrial dysregulation, apoptosis, and endoplasmic reticulum stress, which have important potential for improving oocyte quality and treating female infertility.