Affiliation:
1. Children's Cancer Institute Lowy Cancer Research Centre, UNSW Kensington NSW Australia
2. School of Clinical Medicine UNSW Medicine & Health, UNSW Sydney Kensington NSW Australia
Abstract
AbstractThe conversion of raw sequencing reads to biologically relevant data in high‐throughput single‐cell RNA sequencing experiments is a complex and involved process. Drawing meaning from thousands of individual cells to provide biological insight requires ensuring not only that the data are of the highest quality but also that the signal can be separated from noise. In this article, we describe a detailed analytical workflow, including six pipelines, that allows high‐quality data analysis in single‐cell multiomics. © 2024 The Authors. Current Protocols published by Wiley Periodicals LLC.Basic Protocol 1: Image analysisBasic Protocol 2: Sequencing quality control and generation of a gene expression matrixBasic Protocol 3: Gene expression matrix data pre‐processing and analysisBasic Protocol 4: Advanced analysisBasic Protocol 5: Conversion to flow cytometry standard (FCS) formatBasic Protocol 6: Visualization using graphical interfaces