Combinatorial InVitroFlow‐assisted mutagenesis (CombIMut) yields a 41‐fold improved CelA2 cellulase-Reference-Cited by-同舟云学术

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Combinatorial InVitroFlow‐assisted mutagenesis (CombIMut) yields a 41‐fold improved CelA2 cellulase

Published:2022-05-07 Issue:8 Volume:119 Page:2076-2087
ISSN:0006-3592
Container-title:Biotechnology and Bioengineering
language:en
Short-container-title:Biotech & Bioengineering

Author:

Körfer Georgette¹,Besirlioglu Volkan¹,Davari Mehdi D.²,Martinez Ronny³,Vojcic Ljubica¹⁴,Schwaneberg Ulrich¹⁵^ORCID

Affiliation:

1. Lehrstuhl für Biotechnologie RWTH Aachen University Aachen Germany

2. Department of Bioorganic Chemistry Leibniz Institute of Plant Biochemistry Halle Germany

3. Departamento de Ingeniería en Alimentos Universidad de La Serena La Serena Chile

4. Codexis Inc. Redwood City California USA

5. DWI – Leibniz Institute for Interactive Materials, Forckenbeckstrasse 50 Aachen Germany

Funder

Bundesministerium für Bildung und Forschung

Publisher

Wiley

Subject

Applied Microbiology and Biotechnology,Bioengineering,Biotechnology

Link

https://onlinelibrary.wiley.com/doi/pdf/10.1002/bit.28110

Reference47 articles.

1. Ultrahigh-throughput screening in drop-based microfluidics for directed evolution

2. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs

3. The crystal structure of endoglucanase CelA, a family 8 glycosyl hydrolase from Clostridium thermocellum

4. In vitro compartmentalization by double emulsions: sorting and gene enrichment by fluorescence activated cell sorting

5. Directed Evolution Empowered Redesign of Natural Proteins for the Sustainable Production of Chemicals and Pharmaceuticals

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1. Advances in ultrahigh-throughput screening technologies for protein evolution;Trends in Biotechnology;2023-09

2. Versatile Product Detection via Coupled Assays for Ultrahigh-Throughput Screening of Carbohydrate-Active Enzymes in Microfluidic Droplets;ACS Catalysis;2023-07-21

3. Versatile Product Detection via Coupled Assays for Ultra-high-throughput Screening of Carbohydrate-Active-Enzymes in Microfluidic Droplets;2023-03-29

4. High-throughput screening of microbial strains in large-scale microfluidic droplets;Frontiers in Bioengineering and Biotechnology;2023-03-10

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