Development and characterization of an automated imaging workflow to generate clonally-derived cell lines for therapeutic proteins

Author:

Shaw David1ORCID,Yim Mandy1,Tsukuda Joni1,Joly John C.2,Lin Andy3,Snedecor Brad1,Laird Michael W.1,Lang Steven E.1

Affiliation:

1. Early Stage Cell Culture, Genentech, Inc., 1 DNA Way; South San Francisco CA 94080

2. Analytical Development and Quality Control; Genentech, Inc., 1 DNA Way; South San Francisco CA 94080

3. Pharma Technical Development Project and Portfolio Management; Genentech, Inc., 1 DNA Way; South San Francisco CA 94080

Publisher

Wiley

Subject

Biotechnology

Reference18 articles.

1. Mammalian cell protein expression for biopharmaceutical production;Zhu;Biotechnol Adv,2012

2. Biopharmaceutical benchmarks 2014;Walsh;Nat Biotechnol,2014

3. Quality of biotechnological products: derivation and characterisation of cell substrates used for production of biotechnological/biological products. ICH Harmonised Tripartite Guideline;Dev Biol Stand,1998

4. Guidelines on the quality, safety and efficacy of dengue tetravalent vaccines (live, attenuated) Replacement of Annex 1 of WHO Technical Report Series, No. 932;Biol;WHO Tech Rep Ser,2013

5. Slashing the timelines: opting to generate high-titer clonal lines faster via viability-based single cell sorting;Misaghi;Biotechnol Prog,2016

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