Affiliation:
1. Department of Comparative and Molecular Pathobiology Johns Hopkins School of Medicine Baltimore Maryland USA
Abstract
AbstractExtracellular vesicles (EVs) are intensively investigated for their therapeutic potential and application as drug delivery vehicle. A broad perception of favourable safety profiles and low immunogenicity make EVs an attractive alternative to synthetic nanoparticles. We recently showed that repeated intravenous administration of human cell‐derived EVs into pig‐tailed macaques unexpectedly elicited antibody responses after three or more injections. This coincided with decreasing EV circulation time, and may thus hamper successful EV‐mediated cargo delivery into tissues. Here, we share the custom ELISA protocol that we used to measure such antibody responses. This protocol may help other researchers evaluate immune responses to EV‐based therapies in preclinical studies.
Funder
NIH Office of the Director
National Institute of Allergy and Infectious Diseases
National Institute on Drug Abuse
National Institute of Mental Health
Common Fund