Let‐7a‐5p abrogates progression of papillary thyroid carcinoma cells by decreasing nuclear receptor subfamily 6 group a member 1‐mediated lipogenesis

Abstract

AbstractIncreasing evidence shows that microRNAs (miRNAs) contribute vital roles in papillary thyroid carcinoma (PTC) carcinogenesis, proliferation, invasion, and so on. As the most common endocrine malignancy, there still have largely unknown molecular events. First, our analysis and open access database information indicates that the downregulation of let‐7a‐5p accelerates PTC progression. Next, lentivirus mediates the overexpression of let‐7a‐5p PTC cells, and found let‐7a‐5p suppressed cancer cells proliferation and invasion. Interestingly, bioinformatics analysis hints NR6A1 is the potential target gene of let‐7a‐5p. The regulation was validated by luciferase and quantitative reverse transcription polymerase chain reaction (qRT‐PCR) in PTC tissue and the clinic tumors. Moreover, let‐7a‐5p regulated NR6A1 involved in PTC cells lipogensis in vitro and in vivo. Finally, let‐7a‐5p abrogates PCT xenograft tumors growth, NR6A1 expression and lipogenesis. Taken together, our data indicates that let‐7a‐5p suppresses PCT progression through decreased lipogenesis, the related let‐7a‐5p/NR6A1axis might be promising candidate targets for PTC treatment.