A Convenient Split‐Intein Tag Method for the Purification of Tagless Target Proteins
Author:
Affiliation:
1. Department of Chemical and Biomolecular Engineering, The Ohio State University Columbus Ohio
Publisher
Wiley
Subject
Biochemistry,General Medicine,Structural Biology
Link
https://onlinelibrary.wiley.com/doi/pdf/10.1002/cpps.46
Reference25 articles.
1. Current strategies for the use of affinity tags and tag removal for the purification of recombinant proteins
2. Distribution of split DnaE inteins in cyanobacteria
3. Single-column purification of free recombinant proteins using a self-cleavable affinity tag derived from a protein splicing element
4. Utilizing the C-terminal cleavage activity of a protein splicing element to purify recombinant proteins in a single chromatographic step
5. The potential role of self-cleaving purification tags in commercial-scale processes
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1. Production of native recombinant proteins using a novel split intein affinity technology;Journal of Chromatography A;2024-06
2. A Convenient Self‐Removing Affinity Tag Method for the Simple Purification of Tagless Recombinant Proteins;Current Protocols;2023-10
3. The Inducible Intein-Mediated Self-Cleaving Tag (IIST) System: A Novel Purification and Amidation System for Peptides and Proteins;Molecules;2021-09-30
4. Development of a protein-solubilizing expression method based on the synergistic action of intein ΔI-CM and the solubility enhancer elastin-like polypeptide;Biochemical Engineering Journal;2021-03
5. Opportunities and challenges of the tag-assisted protein purification techniques: Applications in the pharmaceutical industry;Biotechnology Advances;2020-12
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