Affiliation:
1. Laboratory of Gnotobiology Institute of Microbiology of the Czech Academy of Sciences Novy Hradek Czech Republic
2. Laboratory of Microbiome Immunobiology Hirszfeld Institute of Immunology and Experimental Therapy Polish Academy of Sciences Wroclaw Poland
Abstract
AbstractThe currently observed high prevalence of allergic diseases has been associated with changes in microbial exposure in industrialized countries. Defined bacterial components represent a new strategy for modulating the allergic immune response. We show that intranasal administration of exopolysaccharide (EPS) isolated from Lacticaseibacillus (L.) rhamnosus LOCK900 induces TGF‐β1, IgA, and regulatory FoxP3+ T‐cells in the lungs of naïve mice. Using the ovalbumin mouse model, we demonstrate that intranasal administration of EPS downregulates the development of allergic airway inflammation and the Th2 cytokine response in sensitized individuals. At the same time, EPS treatment of sensitized mice, similar to EPS‐induced responses in naïve mice, significantly increased the level of total, OVA‐specific, and also bacteria‐specific IgA in bronchoalveolar lavage and the number of IgA‐producing B‐cells in the lung tissue of these mice. Thus, EPS derived from L. rhamnosus LOCK900 can be considered a safe candidate for preventing the development of allergic symptoms in the lungs of sensitized individuals upon exposure to an allergen.
Funder
Grantová Agentura České Republiky
Subject
Immunology,Immunology and Allergy