Abundant cysteine side reactions in traditional buffers interfere with the analysis of posttranslational modifications and protein quantification - How to compromise
Author:
Affiliation:
1. Core Unit Proteomics, Interdisciplinary Center for Clinical Research, Medical Faculty; University of Münster; Germany
Publisher
Wiley
Subject
Organic Chemistry,Spectroscopy,Analytical Chemistry
Reference35 articles.
1. Surfactant-induced artifacts during proteomic sample preparation;Ji;Anal. Chem.,2015
2. Electrophoresis-related protein modification: Alkylation of carboxy residues revealed by mass spectrometry;Haebel;Electrophoresis,1998
3. An improved trypsin digestion method minimizes digestion-induced modifications on proteins;Ren;Anal. Biochem.,2009
4. Redox biology: Computational approaches to the investigation of functional cysteine residues;Marino;Antioxid. Redox Sign.,2011
5. Diverse functional roles of reactive cysteines;Pace;ACS Chem. Biol.,2013
Cited by 8 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献
1. Comparative Two-Dimensional Fluorescence Gel Electrophoresis;Methods in Molecular Biology;2022-11-16
2. In-solution buffer-free digestion allows full-sequence coverage and complete characterization of post-translational modifications of the receptor-binding domain of SARS-CoV-2 in a single ESI–MS spectrum;Analytical and Bioanalytical Chemistry;2021-11-05
3. Bicarbonate buffers can promote crosslinking and alternative gas-phase dissociation pathways for multiprotein complexes;International Journal of Mass Spectrometry;2021-11
4. In-solution buffer-free digestion for the analysis of SARS-CoV-2 RBD proteins allows a full sequence coverage and detection of post-translational modifications in a single ESI-MS spectrum;2021-05-10
5. Atomic-resolution mapping of transcription factor-DNA interactions by femtosecond laser crosslinking and mass spectrometry;Nature Communications;2020-06-15
1.学者识别学者识别
2.学术分析学术分析
3.人才评估人才评估
"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370
www.globalauthorid.com
TOP
Copyright © 2019-2024 北京同舟云网络信息技术有限公司 京公网安备11010802033243号 京ICP备18003416号-3