Affiliation:
1. College of Biotechnology Jiangsu University of Science and Technology Zhenjiang Jiangsu Province People's Republic of China
2. Jiangsu Province Key Laboratory of Animal Breeding and Molecular Design, College of Animal Science and Technology Yangzhou University Yangzhou Province People's Republic of China
3. Animal Science College Jiangsu Agri‐animal Husbandry Vocational College Taizhou Jiangsu province People's Republic of China
Abstract
AbstractMeiosis, a key step in spermatogenesis, is affected by many factors. Current studies have shown that long noncoding RNAs (lncRNAs) are potential factors regulating meiosis, and their regulatory mechanisms have received much attention. However, little research has been done on its regulatory mechanism in the spermatogenesis of roosters. Here, we found that lncRNA involved in meiosis and spermatogenesis (lncRNA‐IMS) was involved in the regulation of Stra8 by gga‐miR‐31‐5p and hindered the inhibition of Stra8 by gga‐miR‐31‐5p. The acquisition and loss of function experiments demonstrated that lncRNA‐IMS was involved in meiosis and spermatogenesis. In addition, we predicted and determined the core promoter region of lncRNA‐IMS. Prediction of transcription factors, deletion/overexpression of binding sites, knockdown/overexpression of Jun, and dual‐luciferase reporter analysis confirmed that Jun positively activated transcription of lncRNA‐IMS. Our findings further enrich the TF‐lncRNA‐miRNA‐mRNA regulatory network during male meiosis and provide new ideas for studying the molecular mechanism of meiosis and spermatogenesis in chicken spermatogonial stem cells.
Funder
National Natural Science Foundation of China
Subject
Cell Biology,Developmental Biology,Genetics