Affiliation:
1. Key Laboratory of Silkworm and Bee Resource Utilization and Innovation of Zhejiang Province Institute of Applied Bioresource Research College of Animal Science Zhejiang University Hangzhou 310058 P. R. China
2. 3B's Research Group I3Bs – Research Institute on Biomaterials Biodegradables and Biomimetics of University of Minho Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine AvePark, Zona Industrial da Gandra, Barco Guimarães 4805‐017 Portugal
3. Department of Biomedical Engineering The Chinese University of Hong Kong Sha Tin Hong Kong SAR P. R. China
Abstract
Abstract3D in vitro model has emerged as a valuable tool for studying tissue development, drug screening, and disease modeling. 3D systems can accurately replicate tissue microstructures and physiological features, mirroring the in vivo microenvironment departing from conventional 2D cell cultures. Various 3D in vitro models utilizing biomacromolecules like collagen and synthetic polymers have been developed to meet diverse research needs and address the complex challenges of contemporary research. Silk proteins, bearing structural and functional similarities to collagen, have been increasingly employed to construct advanced 3D in vitro systems, surpassing the limitations of 2D cultures. This review examines silk proteins' composition, structure, properties, and functions, elucidating their role in 3D in vitro models. Furthermore, recent advances in biomedical applications involving silk‐based organoid models are discussed. In particular, the unique physiological attributes of silk matrix constituents in in vitro tissue constructs are highlighted, providing a meticulous evaluation of their importance. Additionally, it outlines the current research hurdles and complexities while contemplating future avenues, thereby paving the way for developing complex and biomimetic silk protein‐based microtissues.
Funder
Fundamental Research Funds for the Central Universities