Affiliation:
1. Institute of Biomedical Engineering and Frontier Research Center on Fundamental and Applied Sciences of Matters National Tsing Hua University Hsinchu 300044 Taiwan
2. Department of Chemical Engineering National Tsing Hua University Hsinchu 300044 Taiwan
3. Division of Neurosurgery Department of Surgery MacKay Memorial Hospital Taipei 104217 Taiwan
4. Institute of Nanoengineering and Microsystems National Tsing Hua University Hsinchu 300044 Taiwan
5. Department of Research and Development Win Coat Corporation Hsinchu 30078 Taiwan
Abstract
AbstractMicroneedle (MN) patches, which allow the extraction of skin interstitial fluid (ISF) without a pain sensation, are powerful tools for minimally invasive biofluid sampling. Herein, an MN‐assisted paper‐based sensing platform that enables rapid and painless biofluid analysis with ultrasensitive molecular recognition capacity is developed. First, a controllable‐swelling MN patch is constructed through the engineering of a poly(ethylene glycol) diacrylate/methacrylated hyaluronic acid hydrogel; it combines rapid, sufficient extraction of ISF with excellent structural integrity. Notably, the analyte molecules in the needles can be recovered into a moist cellulose paper through spontaneous diffusion. More importantly, the paper can be functionalized with enzymatic colorimetric reagents or a plasmonic array, enabling a desired detection capacity—for example, the use of paper‐based surface‐enhanced Raman spectroscopy sensors leads to label‐free, trace detection (sub‐ppb level) of a diverse set of molecules (cefazolin, nicotine, paraquat, methylene blue). Finally, nicotine is selected as a model drug to evaluate the painless monitoring of three human volunteers. The changes in the nicotine levels can be tracked, with the levels varying significantly in response to the metabolism of drug in different volunteers. This as‐designed minimally invasive sensing system should open up new opportunities for precision medicine, especially for personal healthcare monitoring.
Subject
Pharmaceutical Science,Biomedical Engineering,Biomaterials
Cited by
5 articles.
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