Surface Functional Modification by Ti3C2Tx MXene on PLLA Nanofibers for Optimizing Neural Stem Cell Engineering

Author:

Zhu Yi‐Dan1,Ma Xi‐Ying2,Li Lin‐Peng1,Yang Quan‐Jun3,Jin Fei2,Chen Zheng‐Nong1,Wu Cui‐Ping1,Shi Hai‐Bo1,Feng Zhang‐Qi2,Yin Shan‐Kai1ORCID,Li Chun‐Yan1

Affiliation:

1. Shanghai Key Laboratory of Sleep Disordered Breathing Department of Otolaryngology‐Head and Neck Surgery Otolaryngology Institute of Shanghai JiaoTong University Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine Shanghai 200233 China

2. School of Chemistry and Chemical Engineering Nanjing University of Science and Technology Nanjing 210094 China

3. Department of Pharmacy Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine Shanghai 200233 China

Abstract

AbstractOptimizing cell substrates by surface modification of neural stem cells (NSCs), for efficient and oriented neurogenesis, represents a promising strategy for treating neurological diseases. However, developing substrates with the advanced surface functionality, conductivity, and biocompatibility required for practical application is still challenging. Here, Ti3C2Tx MXene is introduced as a coating nanomaterial for aligned poly(l‐lactide) (PLLA) nanofibers (M‐ANF) to enhance NSC neurogenesis and simultaneously tailor the cell growth direction. Ti3C2Tx MXene treatment provides a superior conductivity substrate with a surface rich in functional groups, hydrophilicity, and roughness, which can provide biochemical and physical cues to support NSC adhesion and proliferation. Moreover, Ti3C2Tx MXene coating significantly promotes NSC differentiation into both neurons and astrocytes. Interestingly, Ti3C2Tx MXene acts synergistically with the alignment of nanofibers to promote the growth of neurites, indicating enhanced maturation of these neurons. RNA sequencing analysis further reveals the molecular mechanism by which Ti3C2Tx MXene modulates the fate of NSCs. Notably, surface modification by Ti3C2Tx MXene mitigates the in vivo foreign body response to implanted PLLA nanofibers. This study confirms that Ti3C2Tx MXene provides multiple advantages for decorating the aligned PLLA nanofibers to cooperatively improve neural regeneration.

Funder

National Natural Science Foundation of China

Natural Science Foundation of Shanghai Municipality

Publisher

Wiley

Subject

Pharmaceutical Science,Biomedical Engineering,Biomaterials

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