Affiliation:
1. College of Pharmacy China Pharmaceutical University Nanjing 211198 China
2. Nanjing Institute for Food and Drug Control Nanjing 210038 China
3. MOE Joint International Research Laboratory of Animal Health and Food Safety Key Laboratory of Animal Bacteriology Ministry of Agriculture College of Veterinary Medicine Nanjing Agricultural University Nanjing 210095 China
Abstract
AbstractThe healing of biofilm‐infected diabetic wounds characterized by a deteriorative tissue microenvironment represents a substantial clinical challenge. Current treatments remain unsatisfactory due to the limited antibiofilm efficacy caused by weak tissue and biofilm permeability of drugs and the risk of reinfection during the healing process. To address these issues, an integrated therapeutic and preventive nanozyme‐based microneedle (denoted as Fe2C/GOx@MNs) is engineered. The dissolvable tips with enough mechanical strength can deliver and rapidly release Fe2C nanoparticles (NPs)/glucose oxidase (GOx) in the biofilm active regions, enhancing tissue and biofilm permeability of Fe2C NPs/GOx, ultimately achieving highly efficient biofilm elimination. Meanwhile, the chitosan backing layer can not only act as an excellent physical barrier between the wound bed and the external environment, but also prevent the bacterial reinvasion during wound healing with its superior antibacterial property. Significantly, the biofilm elimination and reinfection prevention abilities of Fe2C/GOx@MNs on wound healing are proved on methicillin‐resistant Staphylococcus aureus‐biofilm‐infected diabetic mouse model with full‐thickness wound. Together, these results demonstrate the promising clinical application of Fe2C/GOx@MNs in biofilm‐infected wound healing.
Funder
Natural Science Foundation of Jiangsu Province
National Natural Science Foundation of China
Subject
Pharmaceutical Science,Biomedical Engineering,Biomaterials