Affiliation:
1. State Key Laboratory of Chemical Resource Engineering College of Chemistry Beijing Advanced Innovation Center for Soft Matter Science and Engineering Beijing University of Chemical Technology Beijing 100029 China
2. Department of Chemistry University of Bath Bath BA2 7AY UK
3. School of Chemistry and Chemical Engineering Henan Normal University Xinxiang 453007 China
4. School of Chemistry and Chemical Engineering University of Chinese Academy of Sciences Beijing 100049 China
Abstract
Abstractβ‐amyloid (Aβ) is one of the important biomarkers for diagnosing Alzheimer's disease (AD). Many near‐infrared probes based on the donor‐π‐acceptor structure have been developed to detect Aβ. Most reported Aβ probes are based on the N,N‐dimethylamino group as the ideal donor, which is a widely accepted binding unit. As such, the development of fluorescent probes with improved binding units to detect Aβ is urgently required. Therefore, with this research three anchoring molecular rotor electron donors consisting of cyclic amines of different ring sizes are developed, namely five‐membered ring (TPyr), six‐membered ring (TPip), and seven‐membered ring (THAI). These new anchored molecular rotors are connected to a 4,4‐difluoro‐4‐bora‐3a,4a‐diaza‐s‐indacene (BODIPY) and named TPyrBDP, TPipBDP, and THAIBDP. These probes exhibit high affinities (from 28 to 54 nm) for Aβ1–42 aggregates. The six‐membered ring dye TPipBDP exhibits the highest signal‐to‐noise (75.5‐fold) and higher affinity (28.30 ± 5.94 nm). TPipBDP can cross the blood‐brain barrier and exhibits higher fluorescence enhancement with APP/PS1 (AD) double transgenic (Tg) mice than with wild‐type (WT) mice.
Funder
National Key Research and Development Program of China
China Scholarship Council
Natural Science Foundation of Beijing Municipality
Subject
Pharmaceutical Science,Biomedical Engineering,Biomaterials
Cited by
2 articles.
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