Electrocatalytic Palladium Nanoclusters as Versatile Indicators of Bioassays: Rapid Electroanalytical Detection of SARS‐CoV‐2 by Reverse Transcription Loop‐Mediated Isothermal Amplification

Author:

Rodríguez‐Penedo Alejandro1,Rioboó‐Legaspi Pablo1,González‐López Andrea1,Lores‐Padín Ana1,Pereiro Rosario1,García‐Suárez María del Mar2,Cima‐Cabal María Dolores2,Costa‐Rama Estefanía1,Fernández Beatriz1,Fernández‐Abedul M. Teresa1ORCID

Affiliation:

1. Deparment of Physical and Analytical Chemistry University of Oviedo Oviedo 33006 Spain

2. School of Engineering and Technology International University of La Rioja Logroño 26006 Spain

Abstract

AbstractQuantitative polymerase chain reaction (qPCR) is considered the gold standard for pathogen detection. However, improvement is still required, especially regarding the possibilities of decentralization. Apart from other reasons, infectious diseases demand on‐site analysis to avoid pathogen spreading and increase treatment efficacy. In this paper, the detection of SARS‐CoV‐2 is carried out by reverse transcription loop‐mediated isothermal amplification, which has the advantage of requiring simple equipment, easily adaptable to decentralized analysis. It is proposed, for the first time, the use of palladium nanoclusters (PdNCs) as indicators of the amplification reaction at end point. The pH of the medium decreases during the reaction and, in turn, a variation in the catalytic activity of PdNCs on the oxygen reduction reaction (ORR) can be electrochemically observed. For the detection, flexible and small‐size screen‐printed electrodes can be premodified with PdNCs, which together with the use of a simple and small electrochemical equipment would greatly facilitates their integration in field‐deployable devices. This would allow a faster detection of SARS‐CoV‐2 as well as of other future microbial threats after an easy adaptation.

Publisher

Wiley

Subject

Pharmaceutical Science,Biomedical Engineering,Biomaterials

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