Fabrication and Cell Culture Applications of Core‐Shell Hydrogel Fibers Composed of Chitosan/DNA Interfacial Polyelectrolyte Complexation and Calcium Alginate: Straight and Beaded Core Variations

Author:

Utagawa Yoshinobu1,Ino Kosuke1ORCID,Takinoue Masahiro2ORCID,Shiku Hitoshi13ORCID

Affiliation:

1. Graduate School of Engineering Tohoku University Sendai 980–8579 Japan

2. Department of Computer Science Tokyo Institute of Technology Yokohama 226–8502 Japan

3. Graduate School of Environmental Studies Tohoku University Sendai 980–8579 Japan

Abstract

AbstractCore‐shell hydrogel fibers are widely used in cell culture applications. A simple and rapid method is presented for fabricating core‐shell hydrogel fibers, consisting of straight or beaded core fibers, for cell culture applications. The core fibers are prepared using interfacial polyelectrolyte complexation (IPC) with chitosan and DNA. Briefly, two droplets of chitosan and DNA are brought in contact to form an IPC film, which is dragged to prepare an IPC fiber. The incubation time and DNA concentration are adjusted to prepare straight and beaded IPC fibers. The fibers with Ca2+ are immersed in an alginate solution to form calcium alginate shell hydrogels around the core IPC fibers. To the best of the knowledge, this is the first report of core‐shell hydrogel fibers with IPC fiber cores. To demonstrate cell culture, straight hydrogel fibers are applied to fabricate hepatic models consisting of HepG2 and 3T3 fibroblasts, and vascular models consisting of human umbilical vein endothelial cells and 3T3 fibroblasts. To evaluate the effect of co‐culture, albumin secretion, and angiogenesis are evaluated. Beaded hydrogel fibers are used to fabricate many size‐controlled spheroids for fiber and cloning applications. This method can be widely applied in tissue engineering and cell analysis.

Funder

Japan Agency for Medical Research and Development

Japan Association for Chemical Innovation

Japan Society for the Promotion of Science

Publisher

Wiley

Subject

Pharmaceutical Science,Biomedical Engineering,Biomaterials

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