Porous Photocrosslinkable Hydrogel Functionalized with USC Derived Small Extracellular Vesicles for Corpus Spongiosum Repair

Author:

Wang Lin1234,Yu Mingming15ORCID,Yang Yunlong6,Lv Yiqing1,Xie Hua1,Chen Jiasheng1,Peng Xufeng1,Peng Zhiwei1,Zhou Lijun1,Wang Yang6,Huang Yichen1,Chen Fang123ORCID

Affiliation:

1. Department of Urology Shanghai Children's Hospital School of medicine Shanghai Jiao Tong University Shanghai 200240 China

2. Department of Urology Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine Shanghai 200233 China

3. Shanghai Eastern Institute of Urologic Reconstruction Shanghai 200233 China

4. Department of Urology Renji Hospital School of Medicine Shanghai Jiao Tong University Shanghai 200240 China

5. Department of Ultrasound in Medicine Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine Shanghai 200233 China

6. Institute of Microsurgery on Extremities Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine Shanghai 200233 China

Abstract

AbstractReconstruction of a full‐thickness spongy urethra is difficult because a corpus spongiosum (CS) defect cannot be repaired using self‐healing or substitution urethroplasty. Small extracellular vesicles (sEVs) secreted by urine‐derived stem cells (USC‐sEVs) strongly promote vascular regeneration. In this study, it is aimed to explore whether USC‐sEVs promote the repair of CS defects. To prolong the in vivo effects of USC‐sEVs, a void‐forming photoinduced imine crosslinking hydrogel (vHG) is prepared and mixed with the USC‐sEV suspension. vHG encapsulated with USC‐sEVs (vHG‐sEVs) is used to repair a CS defect with length of 1.5 cm and width of 0.8 cm. The results show that vHG‐sEVs promote the regeneration and repair of CS defects. Histological analysis reveals abundant sinusoid‐like vascular structures in the vHG‐sEV group. Photoacoustic microscopy indicates that blood flow and microvascular structure of the defect area in the vHG‐sEV group are similar to those in the normal CS group. This study confirms that the in situ‐formed vHG‐sEV patch appears to be a valid and promising strategy for repairing CS defects.

Funder

National Natural Science Foundation of China

Publisher

Wiley

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