Affiliation:
1. Key Laboratory of Biomaterials of Guangdong Higher Education Institutes Engineering Technology Research Center of Drug Carrier of Guangdong Department of Biomedical Engineering Jinan University Guangzhou 510632 China
Abstract
AbstractChemodynamic therapy is a promising tumor treatment strategy. However, it remains a great challenge to overcome the unavoidable off‐target damage to normal tissues. In this work, it is discovered that magnetoferritin (M‐HFn, biomimic peroxidase) can form nanocomplexes with glucose oxidase (GOD) in the presence of glucose, thus inhibiting the enzyme activity of GOD. Interestingly, GOD&M‐HFn (G‐M) nanocomplexes can dissociate under near‐infrared (NIR) laser, reactivating the enzyme cascade. Based on this new finding, a spatiotemporally controllable biocatalytic cascade in red blood cell (RBC) nanovesicles (G‐M@RBC‐A) is fabricated for precise tumor therapy, which in situ inhibits enzyme cascade between GOD and M‐HFn during blood circulation and reactivates the cascade activity in tumor site by NIR laser irradiation. In RBC nanovesicles, GOD is grabbed by M‐HFn to form G‐M nanocomplexes in the presence of glucose, thus inhibiting the Fenton reaction and reducing side effects. However, after NIR laser irradiation, G‐M nanocomplexes are spatiotemporally dissociated and the cascade activity is reactivated in the tumor site, initiating reactive oxygen species damage to cancer cells in vivo. Therefore, this work provides new insight into the fabrication of spatiotemporally controllable biocatalytic cascade for precise cancer therapy in the future.
Funder
National Natural Science Foundation of China
Natural Science Foundation of Guangdong Province
Fundamental Research Funds for the Central Universities
Subject
Pharmaceutical Science,Biomedical Engineering,Biomaterials