Affiliation:
1. Center for Reproductive Medicine, Ren Ji Hospital Shanghai Jiao Tong University School of Medicine Shanghai China
2. Shanghai Key Laboratory for Assisted Reproduction and Reproductive Genetics Shanghai China
3. Shandong Provincial Key Laboratory of Animal Resistance Biology, College of Life Sciences Shandong Normal University Jinan Shandong China
Abstract
Abstract5‐Methylcytosine (m5C) is a prevalent RNA modification in messenger RNAs (mRNAs). Despite its abundance, its role in the decidua of pre‐eclampsia (PE) remains elusive. In this study, we utilized methylated RNA immunoprecipitation sequencing (MeRIP‐seq) and RNA‐sequencing (RNA‐seq) to map m5C peaks and mRNA expression profile in the decidua of human early‐onset PE (EPE), late‐onset PE (LPE), and normal pregnancy (NP). Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analyses elucidated potential roles of the differentially methylated mRNAs (DMGs) and differentially expressed mRNAs in decidualization pathways. Integrative analysis of MeRIP‐seq and RNA‐seq data pinpointed 50 candidate genes linked to PE, marked by both differentially methylated m5C peaks and congruent expression changes. To validate these observations, we selected nine genes for verification via quantitative PCR. Our results underscore the precision and reproducibility of our bioinformatics approach. Importantly, we propose that changes in m5C modification and expression of relevant mRNA might influence the pathogenesis of PE by hampering decidualization. This work shines light on the distinct mRNA m5C modification patterns and expression profiles in the decidua of PE, implicating pivotal signaling disruptions and decidualization impediments in the onset of PE.
Funder
National Natural Science Foundation of China
Subject
Cell Biology,Molecular Biology,Biochemistry