Affiliation:
1. Department of Chemistry McGill University 801 Sherbrooke St. West Montreal QC H3A 0B8 Canada
2. Department of Biochemistry and Centre de Recherche en Biologie Structurale McGill University Montreal QC Canada
3. Department of Chemistry University of Rome Tor Vergata Rome Italy
Abstract
AbstractDNA nanotubes (NTs) have attracted extensive interest as artificial cytoskeletons for biomedical, synthetic biology, and materials applications. Here, we report the modular design and assembly of a minimalist yet robust DNA wireframe nanotube with tunable cross‐sectional geometry, cavity size, chirality, and length, while using only four DNA strands. We introduce an h‐motif structure incorporating double‐crossover (DX) tile‐like DNA edges to achieve structural rigidity and provide efficient self‐assembly of h‐motif‐based DNA nanotube (H‐NT) units, thus producing programmable, micrometer‐long nanotubes. We demonstrate control of the H‐NT nanotube length via short DNA modulators. Finally, we use an enzyme, RNase H, to take these structures out of equilibrium and trigger nanotube assembly at a physiologically relevant temperature, underlining future cellular applications. The minimalist H‐NTs can assemble at near‐physiological salt conditions and will serve as an easily synthesized, DNA‐economical modular template for biosensors, plasmonics, or other functional materials and as cost‐efficient drug‐delivery vehicles for biomedical applications.
Funder
Natural Sciences and Engineering Research Council of Canada
Canada Research Chairs
Canada Council for the Arts
Subject
General Chemistry,Catalysis
Cited by
1 articles.
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