A Rapid Sputum‐based Lateral Flow Assay for Airway Eosinophilia using an RNA‐cleaving DNAzyme Selected for Eosinophil Peroxidase

Author:

Ali M. Monsur1,Mukherjee Manali2,Radford Katherine2,Patel Zil2,Capretta Alfredo1,Nair Parameswaran2,Brennan John D.1ORCID

Affiliation:

1. Biointerfaces Institute McMaster University 1280 Main Street West L8S 4K1 Hamilton ON Canada

2. Division of Respirology McMaster University Firestone Institute of Respiratory Health at St. Joseph's Health Care L8N 4A6 Hamilton ON Canada

Abstract

AbstractThe first protein‐binding allosteric RNA‐cleaving DNAzyme (RCD) obtained by direct in vitro selection against eosinophil peroxidase (EPX), a validated marker for airway eosinophilia, is described. The RCD has nanomolar affinity for EPX, shows high selectivity against related peroxidases and other eosinophil proteins, and is resistant to degradation by mammalian nucleases. An optimized RCD was used to develop both fluorescence and lateral flow assays, which were evaluated using 38 minimally processed patient sputum samples (23 non‐eosinophilic, 15 eosinophilic), producing a clinical sensitivity of 100 % and specificity of 96 %. This RCD‐based lateral flow assay should allow for rapid evaluation of airway eosinophilia as an aid for guiding asthma therapy.

Funder

Grand Challenges Canada

Publisher

Wiley

Subject

General Chemistry,Catalysis

Cited by 4 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

1. Human Sputum Proteomics: Advancing Non-Invasive Diagnosis of Respiratory Diseases with Enhanced Biomarker Analysis Methods;International Journal of Translational Medicine;2024-05-31

2. In‐vitro Clinical Diagnostics using RNA‐Cleaving DNAzymes;ChemBioChem;2024-04-29

3. A survey of severe asthma in Canada: results from the CASCADE practice reflective program;Allergy, Asthma & Clinical Immunology;2024-04-18

4. Pulmonology for the rhinologist;Current Opinion in Otolaryngology & Head & Neck Surgery;2023-11-15

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