Affiliation:
1. Faculty of Chemical and Food Technology Ho Chi Minh City University of Technology and Education 1 Vo Van Ngan, Thu Duc City Ho Chi Minh City Vietnam
2. Department of Chemistry University of Houston 4800 Calhoun Rd. TX 77004 Houston USA
Abstract
AbstractStudies of organometallic reactions in living cells commonly rely on ensemble‐averaged measurements, which can obscure the detection of reaction dynamics or location‐specific behavior. This information is necessary to guide the design of bioorthogonal catalysts with improved biocompatibility, activity, and selectivity. By leveraging the high spatial and temporal resolution of single‐molecule fluorescence microscopy, we have successfully captured single‐molecule events promoted by Ru complexes inside live A549 human lung cells. By observing individual allylcarbamate cleavage reactions in real‐time, our results revealed that they occur with greater frequency inside the mitochondria than in the non‐mitochondria regions. The estimated turnover frequency of the Ru complexes was at least 3‐fold higher in the former than the latter. These results suggest that organelle specificity is a critical factor to consider in intracellular catalyst design, such as in developing metallodrugs for therapeutic applications.
Funder
Welch Foundation
National Institute of General Medical Sciences
Subject
General Chemistry,Catalysis
Cited by
2 articles.
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