Monitoring Cardiac Biomarkers with Aptamer‐Based Molecular Pendulum Sensors

Author:

Mahmud Alam1,Chang Dingran2,Das Jagotamoy3,Gomis Surath1,Foroutan Farid4,Chen Jenise B.5,Pandey Laxman2,Flynn Connor D.5,Yousefi Hanie2,Geraili Armin2,Ross Heather J.4,Sargent Edward H.13,Kelley Shana O.23567ORCID

Affiliation:

1. Department of Electrical and Computer Engineering Faculty of Applied Science & Engineering University of Toronto Toronto ON Canada

2. Department of Pharmaceutical Sciences Leslie Dan Faculty of Pharmacy University of Toronto Toronto ON Canada

3. Department of Chemistry Weinberg College of Arts and Sciences Northwestern University Evanston IL USA

4. Ted Rogers Centre for Heart Research Toronto ON Canada

5. Department of Chemistry Faculty of Arts & Science University of Toronto Toronto ON Canada

6. Department of Biomedical Engineering McCormick School of Engineering Northwestern University Evanston IL USA

7. Chan Zuckerberg Biohub Chicago Chicago IL USA

Abstract

AbstractReagent‐free electronic biosensors capable of analyzing disease markers directly in unprocessed body fluids will enable the development of simple & affordable devices for personalized healthcare monitoring. Here we report a powerful and versatile nucleic acid‐based reagent‐free electronic sensing system. The signal transduction is based on the kinetics of an electrode‐tethered molecular pendulum—a rigid double stranded DNA with one of the strands displaying an analyte‐binding aptamer and the other featuring a redox probe—that exhibits field‐induced transport modulated by receptor occupancy. Using chronoamperometry, which enables the sensor to circumvent the conventional Debye length limitation, the binding of an analyte can be monitored as these species increase the hydrodynamic drag. The sensing platform demonstrates a low femtomolar quantification limit and minimal cross‐reactivity in analyzing cardiac biomarkers in whole blood collected from patients with chronic heart failure.

Funder

Canadian Institutes of Health Research

Publisher

Wiley

Subject

General Chemistry,Catalysis

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