Affiliation:
1. College of Engineering and Applied Sciences State Key Laboratory of Analytical Chemistry for Life Science Nanjing University Nanjing 210023 China
2. Department of Laboratory Medicine Nanjing First Hospital Nanjing Medical University Nanjing 210006 China
Abstract
AbstractColonization of cancer cells at secondary sites, a decisive step in tumor metastasis, is strongly dependent on the formation of metastatic microenvironments regulated by intrinsic single‐cell metabolism traits. Herein, we report a single‐cell microfluidic platform for high‐throughput dynamic monitoring of tumor cell metabolites to evaluate tumor malignancy. This microfluidic device empowers efficient isolation of single cells (>99 %) in a squashed state similar to tumor extravasation, and employs enzyme‐packaged metal–organic frameworks to catalyze tumor cell metabolites for visualization. The microfluidic evaluation was confirmed by in vivo assays, suggesting that the platform allowed predicting the tumorigenicity of captured tumor cells and screening metabolic inhibitors as anti‐metastatic drugs. Furthermore, the platform efficiently detected various aggressive cancer cells in unprocessed whole blood samples with high sensitivity, showing potential for clinical application.
Funder
National Key Research and Development Program of China
National Natural Science Foundation of China
Natural Science Foundation of Jiangsu Province
Subject
General Chemistry,Catalysis
Cited by
5 articles.
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