Constructing Photoactivatable Protein with Genetically Encoded Photocaged Glutamic Acid

Author:

Yang Xiaochen1,Zhao Lei1,Wang Ying2,Ji Yanli1,Su Xun‐Cheng1,Ma Jun‐An3,Xuan Weimin12ORCID

Affiliation:

1. State Key Laboratory and Institute of Elemento-Organic Chemistry College of Chemistry Nankai University Tianjin 300071 China

2. School of Life Sciences Tianjin University Tianjin 300072 China

3. Department of Chemistry Frontier Science Center for Synthetic Biology (Ministry of Education) Tianjin University Tianjin 300072 P. R. China

Abstract

AbstractGenetically replacing an essential residue with the corresponding photocaged analogues via genetic code expansion (GCE) constitutes a useful and unique strategy to directly and effectively generate photoactivatable proteins. However, the application of this strategy is severely hampered by the limited number of encoded photocaged proteinogenic amino acids. Herein, we report the genetic incorporation of photocaged glutamic acid analogues in E. coli and mammalian cells and demonstrate their use in constructing photoactivatable variants of various fluorescent proteins and SpyCatcher. We believe genetically encoded photocaged Glu would significantly promote the design and application of photoactivatable proteins in many areas.

Funder

National Natural Science Foundation of China

Publisher

Wiley

Subject

General Chemistry,Catalysis

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