Affiliation:
1. State Key Laboratory of Chemo/Biosensing and Chemometrics College of Chemistry and Chemical Engineering Hunan University Changsha 410082 P.R. China
Abstract
AbstractNear‐infrared region (NIR; 650–1700 nm) dyes offer many advantages over traditional dyes with absorption and emission in the visible region. However, developing new NIR dyes, especially organic dyes with long wavelengths, small molecular weight, and excellent stability and biocompatibility, is still quite challenging. Herein, we present a general method to enhance the absorption and emission wavelengths of traditional fluorophores by simply appending a charge separation structure, dihydropyridopyrazine. These novel NIR dyes not only exhibited greatly redshifted wavelengths compared to their parent dyes, but also displayed a small molecular weight increase together with retained stability and biocompatibility. Specifically, dye NIR‐OX, a dihydropyridopyra‐zine derivative of oxazine with a molecular mass of 386.2 Da, exhibited an absorption at 822 nm and an emission extending to 1200 nm, making it one of the smallest molecular‐weight NIR‐II emitting dyes. Thanks to its rapid metabolism and long wave‐length, NIR‐OX enabled high‐contrast bioimaging and assessment of cholestatic liver injury in vivo and also facilitated the evalua‐tion of the efficacy of liver protection medicines against cholestatic liver injury.