NIR‐II Fluorescence Sensor Based on Steric Hindrance Regulated Molecular Packing for In Vivo Epilepsy Visualization

Author:

Zhao Mengyao1,Lai Weiping2,Li Benhao1,Bai Tianwen2,Liu Chunyan2,Lin Yanfei2,An Shixuan2,Guo Longhua2,Li Lei2,Wang Jianbo2,Zhang Fan1ORCID

Affiliation:

1. Department of Chemistry State Key Laboratory of Molecular Engineering of Polymers and iChem Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials Fudan University Shanghai 200433 China

2. College of Biological Chemical Sciences and Engineering Jiaxing Key Laboratory of Molecular Recognition and Sensing Jiaxing University Jiaxing 314001 China

Abstract

AbstractFluorescence sensing is crucial to studying biological processes and diagnosing diseases, especially in the second near‐infrared (NIR‐II) window with reduced background signals. However, it's still a great challenge to construct “off‐on” sensors when the sensing wavelength extends into the NIR‐II region to obtain higher imaging contrast, mainly due to the difficult synthesis of spectral overlapped quencher. Here, we present a new fluorescence quenching strategy, which utilizes steric hindrance quencher (SHQ) to tune the molecular packing state of fluorophores and suppress the emission signal. Density functional theory (DFT) calculations further reveal that large SHQs can competitively pack with fluorophores and prevent their self‐aggregation. Based on this quenching mechanism, a novel activatable “off‐on” sensing method is achieved via bio‐analyte responsive invalidation of SHQ, namely the Steric Hindrance Invalidation geNerated Emission (SHINE) strategy. As a proof of concept, the ClO‐sensitive SHQ lead to the bright NIR‐II signal release in epileptic mouse hippocampus under the skull and high photon scattering brain tissue, providing the real‐time visualization of ClO generation process in living epileptic mice.

Funder

National Key Research and Development Program of China

National Natural Science Foundation of China

Publisher

Wiley

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