Affinity purification of metalloprotease from marine bacterium using immobilized metal affinity chromatography

Author:

Li Shangyong123,Wang Linna123,Yang Juan123,Bao Jing123,Liu Junzhong123,Lin Shengxiang4,Hao Jianhua123,Sun Mi123

Affiliation:

1. Yellow Sea Fisheries Research Institute; Chinese Academy of Fishery Sciences; Qingdao China

2. Key Laboratory for Sustainable Utilization of Marine Fisheries Resources; Qingdao China

3. Ministry of Agriculture, Qingdao Key Laboratory of Marine Enzyme Engineering; Qingdao China

4. Laboratory of Oncology and Molecular Endocrinology; CHUL Research Center (CHUQ) and Laval University; Quebec Canada

Funder

National Science Foundation-Shandong province Joint Fund

International Science and Technology Cooperation and Exchanges

Postdoctoral Researcher Applied Research Project of Qingdao

National Natural Science Foundation of China

Science and technique plan of Qingdao

Publisher

Wiley

Subject

Filtration and Separation,Analytical Chemistry

Reference26 articles.

1. MMPs and ADAMTSs in intervertebral disc degeneration;Wang;Clin. Chim. Acta,2015

2. MALDI-TOF MS and CD spectral analysis for identification and structure prediction of a purified, novel, organic solvent stable, fibrinolytic metalloprotease from Bacillus cereus B80;Saxena;Biomed. Res. Int,2015

3. Purification and characterization of a new neutral metalloprotease from marine Exiguobacterium sp. SWJS2;Lei;Biotechnol. Appl. Biochem,2015

4. Purification and characterisation of κ-casein specific milk-clotting metalloprotease from Termitomyces clypeatus MTCC 5091;Majumder;Food. Chem,2015

5. Commercial proteases: present and future;Li;FEBS. Lett,2014

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