Transcriptional and post-transcriptional limitations of high-yielding, PEI-mediated transient transfection with CHO and HEK-293E cells
Author:
Affiliation:
1. Laboratory for Cellular Biotechnology (LBTC), École Polytechnique Fédérale de Lausanne (EPFL); CH-1015 Lausanne Switzerland
2. Protein Expression Core Facility (PECF), École Polytechnique Fédérale de Lausanne (EPFL); CH-1015 Lausanne Switzerland
Publisher
Wiley
Subject
Biotechnology
Reference32 articles.
1. Large-scale transfection of mammalian cells for the fast production of recombinant protein;Pham;Mol Biotechnol,2006
2. Recombinant protein production by large-scale transient gene expression in mammalian cells: state of the art and future perspectives;Baldi;Biotechnol Lett,2007
3. Reflections on more than 10 years of TGE approaches;Geisse;Protein Expr Purif,2009
4. Polyethyleneimine-based transient gene expression processes for suspension-adapted HEK-293E and CHO-DG44 cells;Hacker;Protein Expr Purif,2013
5. Rational vector design and multi-pathway modulation of HEK 293E cells yield recombinant antibody titers exceeding 1 g/l by transient transfection under serum-free conditions;Backliwal;Nucleic Acids Res,2008
Cited by 24 articles. 订阅此论文施引文献 订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献
1. Multidose transient transfection of human embryonic kidney 293 cells modulates recombinant adeno‐associated virus2/5 Rep protein expression and influences the enrichment fraction of filled capsids;Biotechnology and Bioengineering;2024-08-23
2. Demonstration of a robust high cell density transient CHO platform yielding mAb titers of up to 2 g/L without medium exchange;Biotechnology Progress;2024-02-08
3. Production and Purification of Adeno-Associated Viral Vectors (AAVs) Using Orbitally Shaken HEK293 Cells;Methods in Molecular Biology;2024
4. Mitigating transcriptional bottleneck using a constitutively active transcription factor, VP16-CREB, in mammalian cells;Metabolic Engineering;2023-11
5. The cell density effect in animal cell-based bioprocessing: Questions, insights and perspectives;Biotechnology Advances;2022-11
1.学者识别学者识别
2.学术分析学术分析
3.人才评估人才评估
"同舟云学术"是以全球学者为主线，采集、加工和组织学术论文而形成的新型学术文献查询和分析系统，可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容，当前同舟云学术共收录了国内外主流学术期刊6万余种，收集的期刊论文及会议论文总量共计约1.5亿篇，并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询！咨询电话：010-8811{复制后删除}0370
www.globalauthorid.com
TOP
Copyright © 2019-2024 北京同舟云网络信息技术有限公司 京公网安备11010802033243号 京ICP备18003416号-3