Synonymous and non‐synonymous codon substitutions can alleviate dependence on GroEL for folding

Author:

Reingewertz Tali Haviv1,Ben‐Maimon Miki1,Zafrir Zohar2,Tuller Tamir23,Horovitz Amnon1ORCID

Affiliation:

1. Department of Chemical and Structural Biology Weizmann Institute of Science Rehovot Israel

2. Department of Biomedical Engineering Tel Aviv University Tel Aviv Israel

3. The Sagol School of Neuroscience Tel‐Aviv University Tel Aviv Israel

Abstract

AbstractThe Escherichia coli GroEL/ES chaperonin system facilitates protein folding in an ATP‐driven manner. There are <100 obligate clients of this system in E. coli although GroEL can interact and assist the folding of a multitude of proteins in vitro. It has remained unclear, however, which features distinguish obligate clients from all the other proteins in an E. coli cell. To address this question, we established a system for selecting mutations in mouse dihydrofolate reductase (mDHFR), a GroEL interactor, that diminish its dependence on GroEL for folding. Strikingly, both synonymous and non‐synonymous codon substitutions were found to reduce mDHFR's dependence on GroEL. The non‐synonymous substitutions increase the rate of spontaneous folding whereas computational analysis indicates that the synonymous substitutions appear to affect translation rates at specific sites.

Publisher

Wiley

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3