Liposomal doxorubicin and free doxorubicin in vivo quantitation method developed on CE‐LIF and its application in pharmacokinetic analysis

Author:

Tie Cai12,Jiang Xiaojuan3,Zhu Chunyan3,Wu Caisheng3ORCID

Affiliation:

1. State Key Laboratory Coal Resources and Safe Mining China University of Mining and Technology‐Beijing Beijing P. R. China

2. School of Chemical and Environmental Engineering China University of Mining and Technology‐Beijing Beijing P. R. China

3. Fujian Provincial Key Laboratory of Innovative Drug Target Research and State Key Laboratory of Cellular Stress Biology School of Pharmaceutical Sciences Xiamen University Xiamen Fujian P. R. China

Abstract

AbstractAs a novel drug delivery system, liposomes were used to improve pharmacokinetics/pharmacodynamics (PK/PD) characters, minimize toxicity, and enhance drug–target selectivity. However, heterogeneity of drug releasing process and liposome itself challenged traditional pharmaceutical analytical techniques, especially in vivo pharmacokinetic studies. In this study, a novel liposomal doxorubicin (L‐DOX) pharmacokinetic analysis strategy was developed with capillary electrophoresis coupled with laser‐induced fluorescence (CE‐LIF) detector. The background electrolyte (BGE) system was composed of borate and sodium dodecyl sulfate (SDS), which was optimized to successfully achieve simultaneous online separation and quantitative analysis of free DOX and liposome‐encapsulated DOX. The method was applied to the in vivo pharmacokinetic study of L‐DOX in rats. The results showed that the concentration of total DOX (T‐DOX) was gradually decreasing, while the concentration of L‐DOX was relatively stable, with a concentration of 31.6 ± 4.8 µg/mL within 24 h. It was the first time to achieve liposomal drugs in vivo analysis with CE‐LIF. CE‐LIF was proved as potential rapidly real‐time analytical methods for liposomal drugs in vivo occurrence monitoring.

Funder

National Natural Science Foundation of China

Publisher

Wiley

Subject

Clinical Biochemistry,Biochemistry,Analytical Chemistry

Cited by 1 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

1. Annual review of capillary electrophoresis technology in 2023;Chinese Journal of Chromatography;2024-04-01

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