Rapid prototyping of polydimethylsiloxane (PDMS) microchips using electrohydrodynamic jet printing: Application to electrokinetic assays

Abstract

AbstractPolydimethylsiloxane (PDMS) based microfluidic devices have found increasing utility for electrophoretic and electrokinetic assays because of their ease of fabrication using replica molding. However, the fabrication of high‐resolution molds for replica molding still requires the resource‐intensive and time‐consuming photolithography process, which precludes quick design iterations and device optimization. We here demonstrate a low‐cost, rapid microfabrication process, based on electrohydrodynamic jet printing (EJP), for fabricating non‐sacrificial master molds for replica molding of PDMS microfluidic devices. The method is based on the precise deposition of an electrically stretched polymeric solution of polycaprolactone in acetic acid on a silicon wafer placed on a computer‐controlled motion stage. This process offers the high‐resolution (order 10 m) capability of photolithography and rapid prototyping capability of inkjet printing to print high‐resolution templates for elastomeric microfluidic devices within a few minutes. Through proper selection of the operating parameters such as solution flow rate, applied electric field, and stage speed, we demonstrate microfabrication of intricate master molds and corresponding PDMS microfluidic devices for electrokinetic applications. We demonstrate the utility of the fabricated PDMS microchips for nonlinear electrokinetic processes such as electrokinetic instability and controlled sample splitting in ITP. The ability to rapid prototype customized reusable master molds with order 10 m resolution within a few minutes can help in designing and optimizing microfluidic devices for various electrokinetic applications.