Affiliation:
1. Chemistry Department Texas A&M University College Station Texas USA
2. Faculty of Science Charles University Prague Czech Republic
Abstract
AbstractIn 1961, Svensson described isoelectric focusing (IEF), the separation of ampholytic compounds in a stationary, natural pH gradient that was formed by passing current through a sucrose density gradient‐stabilized ampholyte mixture in a constant cross‐section apparatus, free of mixing. Stable pH gradients were formed as the electrophoretic transport built up a series of isoelectric ampholyte zones—the concentration of which decreased with their distance from the electrodes—and a diffusive flux which balanced the generating electrophoretic flux. When polyacrylamide gel replaced the sucrose density gradient as the stabilizing medium, the spatial and temporal stability of Svensson's pH gradient became lost, igniting a search for the explanation and mitigation of the loss. Over time, through a series of insightful suggestions, the currently held notion emerged that in the modern IEF experiment—where the carrier ampholyte (CA) mixture is placed between the anolyte‐ and catholyte‐containing large‐volume electrode vessels (open‐system IEF)—a two‐stage process operates that comprises a rapid first phase during which a linear pH gradient develops, and a subsequent slow, second stage, during which the pH gradient decays as isotachophoretic processes move the extreme pI CAs into the electrode vessels. Here we trace the development of the two‐stage IEF model using quotes from the original publications and point out critical results that the IEF community should have embraced but missed. This manuscript sets the foundation for the companion papers, Parts 2 and 3, in which an alternative model, transient bidirectional isotachophoresis is presented to describe the open‐system IEF experiment.
Funder
Agilent Foundation
Grantová Agentura České Republiky
Subject
Clinical Biochemistry,Biochemistry,Analytical Chemistry
Cited by
4 articles.
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