Affiliation:
1. Zhejiang Provincial Key Laboratory of Anesthesiology The Second Affiliated Hospital of Wenzhou Medical University Wenzhou P. R. China
2. Key Laboratory of Drug Monitoring and Control of Zhejiang Province National Anti‐Drug Laboratory Zhejiang Regional Center Hangzhou P. R. China
3. Collaborative Innovation Center of Seafood Deep Processing, Zhejiang Province Joint Key Laboratory of Aquatic Products Processing Institute of Seafood Zhejiang Gongshang University Hangzhou P. R. China
4. Zhejiang Provincial People's Hospital Affiliated People's Hospital of Hangzhou Medical College Hangzhou P. R. China
5. Hangzhou Linping Hospital of Traditional Chinese Medicine Hangzhou P. R. China
6. Zhejiang Engineering Research Center of Advanced Mass Spectrometry and Clinical Application Faculty of Electrical Engineering and Computer Sciences Ningbo University Ningbo P. R. China
7. Department of Hand and Plastic Surgery The Second Affiliated Hospital of Wenzhou Medical University Wenzhou P. R. China
Abstract
AbstractLiver cancer is generally considered the leading cause of cancer deaths worldwide, and hepatocellular carcinoma (HCC) contributes to more than 90% of liver cancers. The altered lipid metabolism for rapid cancer cell growth and tumor formation has been frequently proven. In this study, an ambient ionization mass spectrometry technique, rapid evaporative ionization mass spectrometry (REIMS) using a monopolar electric knife, called iKnife, was systematically optimized and employed for ex vivo analysis of 12 human HCC tumor tissue specimens together with the paired paracancerous tissue (PT) and noncancerous liver tissue (NCT) specimens. Nine free fatty acids and 34 phospholipids were tentatively identified according to their extract masses and/or tandem mass spectra. With the help of statistical methods, 7 free fatty acids and 10 phospholipids were distributed differently in 3 types of liver tissue specimens (95% confidence interval). The box plots showed these characterized lipid metabolites varied in PT, HCC, and NCT. Compared with PT and NCT, the upregulations of four common fatty acids FA 18:0, FA 20:4, FA 16:0, and FA 18:1, together with phospholipids PC 36:1, PE 38:3, PE (18:0/20:4), PA (O‐36:1), PC (32:1), PC 32:0, PE 34:0, and PC (16:0/18:1), were found in HCC specimens. The sensitivity and specificity of the established statistic model for real‐time HCC tumor diagnosis were 100% and 90.5%, respectively. This study demonstrated that the described REIMS technique is a potential method for rapid lipidomic analysis and characterization of HCC tumor tissue.
Funder
National Natural Science Foundation of China
Subject
Clinical Biochemistry,Biochemistry,Analytical Chemistry