Separation mixed semen of two individuals using magnetic beads coupled ABH blood group antibody

Author:

Yao Jun12ORCID,Adnan Atif3ORCID,Wang Hong‐bo4ORCID

Affiliation:

1. Shanghai Key Lab of Forensic Medicine, Key Lab of Forensic Science, Ministry of Justice China (Academy of Forensic Science) Shanghai P. R. China

2. School of Forensic Medicine China Medical University Shenyang P. R. China

3. Department of Forensic Sciences, College of Criminal Justice Naif Arab University for Security Sciences Riyadh Kingdom of Saudi Arabia

4. Department of Anatomy Shenyang Medical College Shenyang P. R. China

Abstract

AbstractIn sexual assault cases, one of the most common samples collected is a mixed semen stain, which is often found on the vagina, female underwear, or bed sheets. However, it is usually difficult to identify the perpetrator based on this sample alone. One technique that has been developed to address this issue is magnetic bead‐based separation. This method involves using modified magnetic microspheres to capture and enrich specific target cells, in this case, sperm cells. In this study, we utilized magnetic beads coupled with ABH blood group antibody to isolate sperm cells from an individual of a single ABO blood type. Subsequently, polymerase chain reaction amplification and capillary electrophoresis were employed to perform the genotyping the short tandem repeat (STR) loci. This approach allows for the identification of different individuals in a mixed seminal stain sample from two individuals, by first separating sperm cells based on ABH antigen differences and subsequently utilizing autosomal STR typing on the enriched single blood group cells.

Publisher

Wiley

Subject

Clinical Biochemistry,Biochemistry,Analytical Chemistry

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