Concanavalin‐A‐Induced Acute Liver Injury in Mice

Author:

Nabekura Tsukasa123ORCID,Matsuo Soichi456ORCID,Shibuya Akira124ORCID

Affiliation:

1. Life Science Center for Survival Dynamics, Tsukuba Advanced Research Alliance (TARA) University of Tsukuba Tsukuba Ibaraki Japan

2. R&D Center for Innovative Drug Discovery University of Tsukuba Tsukuba Ibaraki Japan

3. Division of Immune Response Aichi Cancer Center Research Institute Nagoya Aichi Japan

4. Department of Immunology, Institute of Medicine University of Tsukuba Tsukuba Ibaraki Japan

5. Doctoral Program in Medical Science, Graduate School of Comprehensive Human Sciences University of Tsukuba Tsukuba Ibaraki Japan

6. Department of Advanced Medical Technologies National Cerebral and Vascular Cancer Center Research Institute Suita Osaka Japan

Abstract

AbstractAcute liver injury is a life‐threatening disease. Although immune responses are involved in the development and exacerbation of acute liver injury, the cellular and molecular mechanisms are not fully understood. Intravenous administration of the plant lectin concanavalin A (ConA) is widely used as a model of acute liver injury. ConA triggers T cell activation and cytokine production by crosslinking glycoproteins, including the T cell receptor, leading to the infiltration of myeloid cells into the liver and the subsequent amplification of inflammation in the liver. Thus, the pathogenesis of ConA‐induced acute liver injury is considered a model of immune‐mediated acute liver injury or autoimmune hepatitis in humans. However, the severity of the liver injury and the analyses of immune cells and non‐hematopoietic cells in the liver following ConA injection are significantly influenced by the experimental conditions. This article outlines protocols for ConA‐induced acute liver injury in mice and evaluation methods for liver injury, immune cells, and non‐hematopoietic cells in the liver. © 2024 Wiley Periodicals LLC.Basic Protocol 1: Induction of acute liver injury by ConA injectionBasic Protocol 2: Evaluation of inflammatory cytokines in mouse plasmaBasic Protocol 3: Preparation of liver sections and histological analysis of liver injuryBasic Protocol 4: Preparation of liver immune cellsBasic Protocol 5: Preparation of hepatocytes, endothelial cells, and hepatic stellate cellsBasic Protocol 6: Flow cytometry of immune and non‐hematopoietic liver cellsBasic Protocol 7: Flow cytometric sorting of endothelial cells and hepatic stellate cellsBasic Protocol 8: Quantitative reverse transcription polymerase chain reaction

Funder

Kato Memorial Bioscience Foundation

Life Science Foundation of Japan

Uehara Memorial Foundation

Japan Society for the Promotion of Science

Publisher

Wiley

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